Expertises

Technological Platform on Transcriptomics

The platform's activities draw on a range of molecular biology and bioinformatics skills, to provide a integrated service from RNA sample to analysis report.

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Services

Global transcriptome analysis without a priori :

  • Microarray gene expression chips
  • Preparation of libraries for :
    • Oriented sequencing of polyadenylated RNAs: mRNAseq
    • Rationalized oriented sequencing of polyadenylated RNAs: 3' mRNA-Seq
    • Oriented sequencing of non-polyadenylated RNAs (ribo depletion) : Total RNAseq
    • Sequencing of small non-coding RNAs: miRNA, siRNA, etc... : SmallRNAseq

Targeted transcriptional expression analysis :

  • Automated pipetting and distribution system with defined programs in 384-well plates
  • Custom design of dispensing programs
  • QuantStudio 5 real-time PCR (Polymerase Chain Reaction) system and analysis software

Sample quality control

  • Nucleic acid quality with TapeStation 4200
  • Nucleic acid concentration with DropSense 96 or Nanodrop spectrophotometer

 

instruments

Availability of equipment and tools :

  •  User training :
    • Automated dispenser for 384-well plate preparation
    • QuantStudio 5 real-time PCR (Polymerase Chain Reaction) system
    • TapeStation 4200
    • DropSense 96
    • Nanodrop
  •   MATRIX web application to explore transcriptomic analysis results https://matrix.toulouse.inrae.fr

Research & Development

  • Automation of protocols and purifications
  • Miniaturization of RNAseq library preparation
  • Customizable protocols for specific needs

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