Seminar Brice Ronsin, le 04 Avril 2025

After serving as technical manager of the electron microscopy centre at the Faculty of Poitiers, Brice Ronsin joined the CNRS in 2001 following a competitive examination for research engineer at the Centre for Developmental Biology in Toulouse (CBD). For 15 years, he was head of the photonic imaging platform, where he was responsible for user training and managing the equipment (three confocal microscopes, one two-photon microscope and one wide-field microscope).

In 2016, the CBD merged with five other units to form the Toulouse Centre for Integrative Biology (CBI). The various imaging platforms merged and pooled their equipment to form the LITC (Light Imaging Toulouse CBI). He is one of three engineers responsible for the technical aspects of the department, specifically wide-field and confocal microscopes, optomanipulation, the Fablab section and prototyping.

Brice will present: a history of microscopy, a review of some physics concepts, the principle of image formation in a microscope, descriptions of the components of a microscope (objectives, chromatic aberration, spherical aberration), resolution in photonic microscopy, the principle of numerical aperture, a description of the condenser and Köhler adjustment, and finally the principle of contrast methods (phase contrast and Nomarski).